Microbiological Assay of Vitamin B6 by Thin-Layer Cup-Plate Method with Saccharomyces carlsbergensis
نویسندگان
چکیده
منابع مشابه
Liquid nitrogen preservation of Saccharomyces carlsbergensis and its use in a rapid biological assay of vitamin B6 (pyridoxine).
Growth medium as well as freezing menstruum greatly influenced the recovery of Saccharomyces carlsbergensis when it was quickly frozen in liquid nitrogen at - 196 C and quickly thawed at 40 C. Nearly 90% recovery in viability was obtained when S. carlsbergensis was grown in Trypticase Soy Broth and frozen in vitamin B(6) basal assay medium. The growth phase of S. carlsbergensis also influenced ...
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Active transport of pyridoxine, pyridoxal, and pyridoxamine occurs in resting cells of Saccharomyces carlsbergensis 4228 and can lead to intracellular concentrations of free vitamin much higher than those supplied externally. The initial Km for pyridoxine uptake is 3.6 x 10(-7) M at 30 degrees and pH 4.5, which are optimum for growth. Transport is inhibited by many unphosphorylated vitamin anal...
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ژورنال
عنوان ژورنال: THE JOURNAL OF VITAMINOLOGY
سال: 1972
ISSN: 0022-5398
DOI: 10.5925/jnsv1954.18.90